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. 2013 May;194(1):81–89. doi: 10.1534/genetics.112.148882

Figure 2.

Figure 2

CI66 contains the K10L2 TR-1-rich knob. (A) Mitotic chromosomes from the inbred CI66. This line contains two copies of a variant of chromosome 10 with a large TR-1-rich knob on the long arm (boxed). TR-1 repeats are in red, 180-bp repeats are in green, and centromeres (the CentC repeat) are in yellow. (B) The landrace PI 490831 segregates for a chromosome that appears similar to chromosome 10 in CI66. The individual shown was heterozygous for the chromosome. (C) A meiotic chromosome in the pachytene substage of meiosis showing the knob previously described as K10L2 (McClintock et al. 1981). Note that the knob appears to be composed of two smaller knobs. This image was prepared using classical acetocarmine methods. (D) Pachytene chromosome 10 from CI66 showing a structure similar to K10L2. (E) Pachytene chromosome from the landrace NSL 2841 showing a similar structure. The images in D and E show DNA staining only (DAPI), but FISH analysis of similar slides confirmed that K10L2 contains TR-1 and no evidence of the knob-180 repeat.