Figure 2. P-lig expression can support recruitment of Th1 cells into the inflamed colon but not into the inflamed small intestine.

A) Naive T cells from wiltype (WT) and Fut7^−/− mice were differentiated in vitro under Th1 polarizing conditions in the absence of RA. P-lig and PMA/ionomycin-induced IFNγ expression of Th1 cells generated from Fut7^−/− and WT mice is shown. B) ⁵¹Cr-labeled Th1 cells generated from WT or Fut7^−/− mice were transferred into naive mice or mice infected orally with 100 cysts of T. gondii three, five or seven days before. Radioactivity (mean ± SD ) per organ was determined three hours after transfer of radioactive T cells and is given as the percentage of total recovered radioactivity (n = 5 animals/group). The experiment was repeated on d7 with 5 animals/group with similar results. C) ⁵¹Cr-labeled Th1 cells from WT or Fut7^−/− mice were injected into SCID mice with established colitis induced by transfer of CD4⁺CD45Rb^high cells. The percentage (mean and SD) of recovered radioactivity per organ of one representative experiment out of 3 independent experiments with 4–6 mice/ group is shown. **p<0.01; Mann Whitney U test.