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. 2013 Apr 22;8(4):e62441. doi: 10.1371/journal.pone.0062441

Figure 4. Lentinan suppresses cell surface levels of TNFR1 in Caco-2 cells.

Figure 4

Lentinan (500 µg/ml) was added into the apical compartment of Caco-2/RAW264.7 co-culture model for 30 min. Subsequently, Caco-2 cells were harvested, fixed, and stained according to the method described in materials and methods. (A) Surface levels of TNFR1 were analyzed by flow cytometry. (B) The resulting gMFIs were plotted as percentages of the gMFI obtained from medium treated cells, with the following fomula: % surface TNFR1 = (lentinan gMFI – isotype control gMFI)/(medium gMFI – isotype control gMFI) ×100. The values represent the means ± SE. Experiments were repeated for three times in triplicate.