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. 2013 Apr 22;8(4):e62441. doi: 10.1371/journal.pone.0062441

Figure 6. Lentinan inhibits TNFR1 protein and mRNA expression in Caco-2 cells.

Figure 6

(A) Lentinan (500 µg/ml) was added into the apical compartment of Caco-2/RAW264.7 co-culture model for 3 h. Subsequently, LPS was added to the basolateral compartment at a concentration of 1 ng/ml, followed by incubation for an additional 2 h. Then, Western blot analysis of TNFR1 was performed on total cell extracts from Caco-2 cells. (B) Lentinan (500 µg/ml) was added into the apical compartment of Caco-2/RAW264.7 co-culture model for 2 h. The TNFR1 mRNA expression in Caco-2 cells was determined by quantitative RT-PCR. The values represent the means ± SE. Experiments were repeated for three times in triplicate. **P<0.01.