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. 2013 Apr 22;8(4):e62441. doi: 10.1371/journal.pone.0062441

Figure 8. Effect of anti-lentinan polyclonal Ab on lentinan inhibition of IL-8 mRNA expression in Caco-2 cells.

Figure 8

A rabbit polyclonal anti-lentinan Ab was diluted with PBS at ratios of 1∶5 or 1∶100, and then mixed with lentinan solution and incubated on ice for 30 min. A rabbit polyclonal isotype control Ab was used as control at the same protein concentration as anti-lentinan Ab (dilution ratio of 1∶5). Antibody-treated lentinan (500 µg/ml) was added into the apical compartment of a co-culture model for 3 h. Subsequently, LPS was added to the basolateral compartment at a concentration of 5 ng/ml, followed by incubation for an additional 3 h. IL-8 mRNA expression in Caco-2 cells was determined by quantitative RT-PCR. (B) TNF-α production in the basolateral compartment was determined by a L929 cytotoxicity assay. The values represent the means ± SE. Experiments were repeated for three times in triplicate. *P<0.05, **P<0.01 vs. LPS control.