Figure 4. Md-fru but not Md-dsx is spliced in the female mode in Md-tra^man2 males.

(A) Md-fru splicing: sex-specific primer pairs were used to detect Md-fru^F transcripts (Md-fru-18 and Md-fru-1) and Md-fru^M transcripts (Md-fru-27 and Md-fru-1) in wildtype XX females, in XY males, in XX; Md-tra^man2 males and in XX; Md-tra^man2 males in which Md-tra was silenced by embryonic dsRNA injections (RNAi Md-tra). As an internal control CYP6D3 transcripts were amplified from the same RNA samples. In XX; Md-tra^man2 males the splicing mode of Md-fru P1 transcripts is predominantly female whereas after silencing of Md-tra it shifts back into the functional male mode. (B) Md-dsx splicing: sex-specific primer pairs were used to detect Md-dsx^F transcripts (Md-dsx-6S and Md-dsx-11AS) and Md-dsx^M transcripts (Md-dsx-6S and Md-dsx-46AS) in wildtype XX females, in XY males and in XX; Md-tra^man2 males. Primer sequences are published in [28]. In contrast to Md-fru, Md-dsx is almost exclusively spliced in the male mode in Md-tra^man2 males.