Figure 4. Phenotypic analysis of seHAdV-T-cells.

A) Percentage values of different cell populations (as indicated, including cytokine-induced killer cells (CIK)) within PBMCs, before (day 0) and after expansion (day12) of 8 donors. Percentage values of TCM (upper left), Naïve (upper right), TEM (lower left) and TEMRA (lower right) T cell populations within CD8⁺ (B), CD4⁺ C), and HAdV-streptamer⁺ T-cells D) on days 0 (white bars) and 12 (black bars). The graph shows mean+SEM of 8 (for CD4⁺ and CD8⁺ T-cells) and 3 (for HAdV-streptamer⁺ T-cells) donors. Notably, for phenotypic analysis of HAdV-streptamer⁺ T cells on day 0 (white bars), beads-based magnetic isolation was performed. Representative dot plots are shown.