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. 2013 Apr 22;8(4):e61255. doi: 10.1371/journal.pone.0061255

Figure 8. E. faecalis MN1 low molecular weight fraction inhibits TSST-1-induced T cell proliferation.

Figure 8

E. faecalis MN1 supernate was treated with ethanol (80% final concentration), insoluble material was removed by centrifugation, soluble material (referred to as the low molecular weight fraction) was concentrated 480 times relative to the original culture fluid, and dilutions incubated with human PBMCs and TSST-1 (1 µg/well) in a superantigenicity assay to assess the ability of secreted factors to inhibit TSST-1-induced T cell proliferation. The PBMC control shows the average CPMs for PBMCs alone and TSST-1 shows the average CPMs for TSST-1 alone. Treatments 48× to 0.0048× were significantly different from TSST-1 alone by individual Student's t tests (*p<0.001). Three independent wells for each treatment were assayed for cell viability, and viability in all cases was >95%.