Figure 5. Physicochemical characterization of reconstituted CSNC+ freeze dried under different conditions.

Particle size recovery after freeze-drying and reconstitution of CSNC+ using different cryoprotectants. (A) Particle size after reconstitution of freeze-dried formulation using sucrose (blue columns) and trehalose (yellow columns) as cryoprotectants at different concentrations. (B) Detailed physicochemical characterization of dried formulations in the presence of sucrose or trehalose (both at 5%) compared to the original characteristics of the suspension (before). Size is shown in yellow columns and polydispersity index (PdI) in black line. Results are presented as mean ± SD.