Figure 2. Lymph node cells from WT and AID^−/− mice produce equivalent levels of cytokines in response to immunization with rhMOG.

WT and AID^−/− mice were immunized with recombinant human MOG (rhMOG) and draining axillary and brachial lymph nodes were harvested after 7 days post-immunization. Four million lymph node cells were plated along with 20 µg of rhMOG. Cultures were kept for 48 hours after which the supernatant was harvested and evaluated for cytokine production by ELISA. The limit of detection of the assay is 125 pg/ml for the IFNγ ELISA and 63 pg/ml for the IL-17 ELISA. Five mice per group were assessed.