Figure 3. Impaired accumulation of cytokine producing CD4⁺ T cells in the CNS of AID^−/− mice.

WT and AID^−/− mice were immunized with recombinant human MOG (rhMOG) and spinal cords (A) and brains (B) were extracted and processed at day 15: the peak of disease. Leukocytes were stimulated ex vivo with PMA/Ionomycin, and brefeldin A was added in the last 4 hours. Cells were then subjected to surface and intracellular cytokine staining. This experiment was performed twice with similar results and 6 mice per group were tested in this experiment. Representative FACS analysis can be seen in Supplemental Figure S2A.