Figure 6. 1D-PAGE resolution of in vitro aged and methylated synucleins, and methyl ester autoradiography.
Synuclein proteins were methylated with 3H-SAM using exogenous PIMT, resolved by 1D PAGE and proteins stained with silver (left panels), or proteins transferred to PVDF and the level of isoaspartate methylation visualised by autoradiography (right panels). Wild-type and mutant α-synuclein proteins formed stable protein oligomers that contained isoaspartate peptide linkages (marked with arrowheads).