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(a) A mixture of t-Asp and bradykinin is placed on swollen brush and a conventional MALDI plate. (b) After 3 min fractionation time, t-Asp is adsorbed by the brush and the eluent containing the unbound bradykinin is pipetted off onto a MALDI plate. (c) The brush is collapsed by treatment with 10% ammonium hydroxide, which expelled the t-Asp that is analyzed on a MALDI plate.
