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. Author manuscript; available in PMC: 2013 Apr 22.

Published in final edited form as: J Neurogenet. 2009;23(4):378–394. doi: 10.3109/01677060903063026

Supernumerary ejps at physiological concentrations of Ca²⁺. A. Different types of ejps triggered by high-frequency nerve stimulation (10 and 15 Hz) in saline containing low (0.2 mM, left column) or physiological (1.0 mM, right column) concentrations of Ca²⁺ in WT larvae. NMJs displaying normal ejps (a, group-reared larvae) or giant ejps with a notch in the rising phase (arrow head in b, isolation-reared larvae) in low-Ca²⁺ saline produced large ejps with a normal waveform when Ca²⁺ was increased to 1.0 mM (d, e). NMJs displaying multi-peak ejps in low-Ca²⁺ saline (c, isolation-reared larvae) retained supernumerary discharges when Ca²⁺ was raised to a physiological concentration (f). Horizontal dashed lines indicate resting membrane potential and vertical dotted lines mark the peaking time of normal ejps. , nerve stimulation. B. Fraction of NMJs displaying supernumerary ejps at 1 mM Ca²⁺ associated with different genotypes and rearing conditions. *, p < 0.05 (X²-test). Number of NMJs in parentheses.

Inline graphic — Supernumerary ejps at physiological concentrations of Ca²⁺. A. Different types of ejps triggered by high-frequency nerve stimulation (10 and 15 Hz) in saline containing low (0.2 mM, left column) or physiological (1.0 mM, right column) concentrations of Ca²⁺ in WT larvae. NMJs displaying normal ejps (a, group-reared larvae) or giant ejps with a notch in the rising phase (arrow head in b, isolation-reared larvae) in low-Ca²⁺ saline produced large ejps with a normal waveform when Ca²⁺ was increased to 1.0 mM (d, e). NMJs displaying multi-peak ejps in low-Ca²⁺ saline (c, isolation-reared larvae) retained supernumerary discharges when Ca²⁺ was raised to a physiological concentration (f). Horizontal dashed lines indicate resting membrane potential and vertical dotted lines mark the peaking time of normal ejps. , nerve stimulation. B. Fraction of NMJs displaying supernumerary ejps at 1 mM Ca²⁺ associated with different genotypes and rearing conditions. *, p < 0.05 (X²-test). Number of NMJs in parentheses.