Figure 8.

Acute effects of a reducing agent, dithiothreitol (DTT), on WT ejps at a physiological concentration of Ca²⁺ (1 mM). A. Acute DTT treatment (~30 min) resulted in supernumerary ejps (b) or ejps with a hump (c). B. Supernumerary ejps (bottom trace) temporarily correlated with motor axon repetitive firing (top trace) indicates increased nerve excitability induced by DTT treatment. C. Muscle regenerative potential manifested as a hump superimposing on the ejp. The hump was inhibited by hyperpolarizing current injection and promoted by depolarizing current injection (superimposed traces). Current injection onset is indicated by the superimposed schematic traces (bottom). ●, nerve stimulation. The DTT effects were similar in both isolation- and group-reared WT larvae.