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. 2013 May 1;319(8):1198–1212. doi: 10.1016/j.yexcr.2013.02.005

Fig. 5.

Fig. 5

Analysis of Invadolysin by immunoblotting. (A) Immunoblot analysis on zebrafish extracts obtained at 0–120 hpf probed with an antibody raised against a peptide specific to zebrafish invadolysin. (B) Immunoblot analysis on zebrafish larval extracts obtained at 0–120 hpf probed with an antibody raised against the C-terminal half of human invadolysin. (C) Immuno-blot analysis on zebrafish larval extracts obtained from non-injected (N), Control (C), ATG-MO (A), and Ex6-MO (6) injected embryos at 24, 48 and 72 hpf, probed with zebrafish invadolysin antibody. (D) Immunoblot analysis on zebrafish extracts obtained from non-injected (N), ATG MO injected exhibiting low, medium and high amount of morpholino fluorescence (ATG-MO), decreasing amounts of injected RNA alone (R1 & R2), or co-injected with ATG-MO and invadolysin transcript (M+R1/R2). Molecular weight markers are shown in kDa, red asterisks denote bands of 74, 55 and 22 kDa.