Figure 1. Immunogenicity of GST-PbMSP1₁₉ immunization.

BALB/c mice (n = 5) were immunized with five doses of 20 μg GST-PbMSP1₁₉ protein. Doses were administered at week 0 s.c. in CFA, at week 3 s.c. in IFA, at weeks 6 and 8 i.p. in IFA, and at week 9 i.p. in PBS. (a) Total IgG responses against recombinant GST-PbMSP1₁₉ protein were measured by ELISA in the serum of mice taken at weeks 5, 9 and 11. Median and range of the titers are shown. The dashed line indicates the lower limit of detection. ** P < 0.01 by Friedman test with Dunn's correction for comparison of all time-points. (b) P. berghei ANKA schizonts were fixed with 4% formaldehyde and permeabilized with 0.1% NP40. Pooled sera from week 11 in (A) were tested at a dilution of 1:100. Naïve pre-immunization serum was used as a negative control. Bound antibodies were detected using goat anti-mouse IgG conjugated to Alexa Fluor 488. Images show left to right the visible schizonts, followed by fluorescence (green) and DAPI staining (blue).