Figure 2. Immunogenicity of AdHu5-MVA viral vector vaccines.

(a) BALB/c mice (n = 5) were immunized i.d. with 1 × 10¹⁰ vp AdHu5 and boosted eight weeks later i.d. with 1 × 10⁷ pfu MVA expressing PbMSP1₄₂. Total IgG responses against recombinant GST-PbMSP1₁₉ protein were measured by ELISA in the serum of mice taken at weeks 2, 8 and 10. Median and range of the titers are shown. The dashed line indicates the lower limit of detection. * P < 0.05 by Friedman test with Dunn's correction for comparison of all time-points. (b) P. berghei ANKA schizonts were fixed with 4% formaldehyde and permeabilized with 0.1% NP40. Sera from mice immunized with 1 × 10¹⁰ vp AdHu5 and boosted with 1 × 10⁷ pfu MVA expressing the indicated antigens were used at a dilution of 1:100. Bound antibodies were detected using goat anti-mouse IgG conjugated to Alexa Fluor 488. Images show fluorescence (green) and DAPI staining (blue).