Figure 7. Efficacy of AdHu5-MVA PbMSP1₄₂ in CD32b^−/− mice.

CD32b^−/− mice were immunized i.d. with 1 × 10¹⁰ vp AdHu5 and boosted eight weeks later i.d. with 1 × 10⁷ pfu MVA encoding either no antigen (Control) or PbMSP1₄₂. (a) On days 14, 54 (pre-boost) and 70 (2 weeks post-boost) sera were collected and PbMSP1₁₉-specific total IgG measured by ELISA. Dots represent individual mice and bars show median endpoint titre. * P = 0.02 between d54 and d70 by Wilcoxon matched pairs signed rank test. The dashed line indicates the lower limit of detection. Two weeks post-boost (b) PbMSP1₄₂ immunized CD32b^−/− mice and (c) control immunized mice were challenged with 5 × 10² P. berghei ANKA pRBC and monitored for parasitemia. Groups of (d) non-immunized (naïve) CD32b^−/− and (e) wild-type C57BL/6 mice were also challenged. Lines represent individual mice of 5–7 mice/group (females solid lines and males dashed lines). † indicates that mice were culled. The number culled that day from the total number of mice in the group is shown in brackets.