Table 1.
Effective naproxen concentration for reduction of MDCK cell viral titersa
| [Naproxen] (μM) | Method | Time p.i. (h) | MOI | EC50 (μM ± SD) | CC50 (μM ± SD) | SI |
|---|---|---|---|---|---|---|
| 0–10 | MTT (n = 3) | 24 | 1,119 ± 320 | |||
| 48 | 1,435 ± 550 | |||||
| 72 | 2,167 ± 600 | |||||
| 0–10 | Trypan blue (n = 2) | 48 | 1,700 ± 900 | |||
| Mean | 1,605 ± 328 | |||||
| 0–500 | MTT (n = 3) | 48 | 10−3 WSN/33 | 15 ± 5 | ||
| 0–500 | Viral titers (n = 4) | 24 | 10−3 WSN/33 | 20 ± 7 | ||
| 48 | 11 ± 4 | |||||
| 72 | 18 ± 5 | |||||
| 0–500 | Viral RNA (n = 2) | 48 | 10−3 WSN/33 | 16 ± 5 | ||
| Mean | 16 ± 5 | 100 ± 30 | ||||
| 0–500 | Viral titers (n = 2) | 48 | 10−2 WSN/33 | 25 ± 7 |
a
Data in columns 5, 6, and 7 represent EC50 (cellular toxicity of naproxen on uninfected MDCK cells under the same cell growth conditions), CC50, and selectivity index (SI [CC50/EC50]) values, respectively.