Table 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Relevant characteristics or sequence (5′–3′)a | Source or reference |
|---|---|---|
| Strains | ||
| PT5 | PAO1, mexT nonfunctional | Laboratory collection |
| PAO1-FV | PAO1, mexT functional | M. Camara (Nottingham, United Kingdom) |
| LESB58 | Lacks piuA | C. Winstanley (Liverpool, United Kingdom) |
| 39016 | Lacks piuA | C. Winstanley (Liverpool, United Kingdom) |
| MPAO1fecA | fecA (PA3901)::TnphoA, Tetr | 24 |
| MPAO1nirJ | nirJ::TnlacZ, Tetr | 24 |
| MPAO1piuA | piuA (PA4514)::TnlacZ, Tetr | 24 |
| MPAO1piuC | piuC (PA4515)::TnlacZ, Tetr | 24 |
| MPAO1fecI | fecI (PA3899)::TnlacZ, Tetr | 24 |
| MPAO1femA | femA (PA1910)::TnlacZ, Tetr | 24 |
| MPAO1fiuA | fiuA (PA0470)::TnlacZ, Tetr | 24 |
| MPAO1fiuA | pfuA (PA1322)::TnlacZ, Tetr | 24 |
| PAO1-furA4 | Fur H86Y, constitutive siderophore expression | 29 |
| Plasmids | ||
| pIApX2 | Broad-host-range expression vector, Apr | I. Attree (Grenoble, France) |
| pfecI | fecI PCR fragment of PT5 cloned in pIApX2, Apr | This study |
| pfecA | fecA PCR fragment of PT5 cloned in pIApX2, Apr | This study |
| Primers | ||
| FecI-Bam | CCGGGATCCTAATCCTTCTGCTCCGGGAAT | This study |
| FecI-Hind | CCGAAGCTTACGGCCTGTTCAGCCACCTG | This study |
| FecA-Bam | CCGGGATCCGTGCTACCTGTGGAGGTACGG | This study |
| FecA-Hind | CCGAAGCTTGCTGCTGATGAAACAGTTGGAG | This study |
a
Restriction sites in the primer sequences are underlined. Tetr, tetracycline resistance; Apr, ampicillin resistance.