Table 2.

MICs for PAO1 and mutants selected on BAL30072

Strain	Growth mediuma	Selective agent (μg/ml)b	MIC (μg/ml)c				Relative nitrocefin hydrolysis
			BAL	ATM	MEM	CIP
PAO1	NA	NA	1	4	1	0.125	1.0
PAO1-M1	IST + BPL	BAL (20)	32	>32	1	0.125	5.1
PAO1-M3	IST + AFC	BAL (20)	32	>32	1	0.125	7.3
PAO1-M9	IST + BPL	BAL (25)	>32	>32	1	0.125	16
PAO1-M18	IST + AFC	BAL (25)	>32	>32	1	0.125	19
BAL5	LB	BAL (4)	16	4	1	0.125	0.7
BAL6	LB	BAL (4)	16	4	1	0.125	0.9
BAL13	LB	BAL (8)	16	4	1	0.125	0.8
BAL14	LB	BAL (8)	16	4	1	0.125	1.1
PAO1-FV	NA	NA	16	4	2	0.25	1.2
PAO1-FV16	IST + BPL	BAL (75)	>64	32	2	0.25	8.1
PAO1-FV25	IST + BPL	ATM (8)	16	32	16	1	1.9

^aIST, IsoSensiTest broth; BPL, 2,2′-bipyridyl; AFC, ammonium ferricitrate; NA, not applicable.

^bNumbers in parentheses indicate the BAL30072 concentrations in μg/ml used for the selection of mutants.

^cBAL, BAL30072; ATM, aztreonam; MEM, meropenem; CIP, ciprofloxacin.