Fig 4.

Levels of AFN-1252 in serum and pouch fluid following a single oral dose. S. aureus Wood46-infected animals received a single oral dose of AFN-1252 (100 mg/kg) 2 h after infection (time zero). At the indicated times, blood plasma and pouch fluid were sampled and AFN-1252 levels were determined in plasma (▲) and in the pouch exudate (●) by LC-MS. Mean values represent the plasma and pouch fluid levels of 3 to 9 samples harvested at the indicated times after AFN-1252 dosing. A total of 3 replicates were analyzed for each sample by LC-MS, and the limit of detection for the assay was 0.01 μg/ml. Error bars indicate standard deviations. AFN-1252 levels at 48 h were below detection.