Table 2.
Profile of resistance to DCV in the in vitro replicon system: 1b replicona
| Mutation | Replication level (%) |
EC50 (ng/ml)b |
Fold resistance | ||
|---|---|---|---|---|---|
| Avg | SD | Avg | SD | ||
| None (WT) | 100 | 0.0019 | 0.0002 | 1 | |
| L31I | 54 | 12 | 0.0027 | 0.0011 | 1.4 |
| P32del | 29.1 | 3.2 | >741 | >390,000 | |
| L31I-Y93H | 43 | 11 | 4.8 | 2.5 | 2,526 |
| P32del-Y93H | No replication | ND | |||
a
The GT-1b replicon is Con1 with cell culture replication-enhancing mutation S2204I. Additional GT-1b R30Q, L31M, L31V, F37L, Q54H, Q54N, Y93C, Y93H, L31M-Y93H, L31V-Y93H, Q54H-Y93H, and L31V-Q54H-Y93H variants have been previously reported (1, 2, 4, 9) and are not included in the table. SDs are for ≥3 replicates.
b
1 ng/ml = 1.35 nM. An EC50 of <10 nM indicates low resistance, an EC50 of 10 to 100 nM indicates moderate resistance, and an EC50 of >100 nM indicates high resistance.