Table 1.

Phenotypic and genotypic features of the 17 imipenem-resistant P. aeruginosa clinical isolates^a

Strain	Date of isolation (day/mo/yr)	Patient age	Sex	Source	IMP MIC (μg/ml)	Resistance phenotype								VIM-2 production	Sequence type
						TIC	TCC	TZP	CAZ	GEN	CIP	FOS	COL
1	15/12/2010	42 yr	M	Bronchial aspirate	12	R	R	R	R	R	S	S	S	+	ST1420
2	15/12/2010	18 yr	F	Blood	32	R	R	R	R	R	S	S	S	+	ST1420
3	28/12/2010	18 mo	M	Bronchial aspirate	12	R	R	R	R	R	S	S	S	+	ST162
4	25/02/2011	2 mo	M	Bronchial aspirate	8	R	R	R	R	R	R	R	S	+	ST1420
5	27/02/2011	7 yr	M	Bronchial aspirate	16	R	R	R	R	R	R	R	S	+	ST1420
6	08/03/2011	7 days	M	Bronchial aspirate	32	R	R	R	R	S	S	R	S	+	ST1420
7	22/03/2011	57 yr	M	Urinary catheter	12	R	R	R	R	R	R	S	S	+	ST1420
8	23/03/2011	43 yr	M	Urinary catheter	32	R	R	R	R	S	S	R	S	+	ST1420
9	18/04/2011	35 yr	M	Urine	12	R	R	R	R	S	S	R	S	+	ST1420
10	25/05/2011	7 yr	M	Pus	16	R	R	R	R	R	S	R	S	+	ST1420
11	19/06/2011	46 yr	M	Urine	16	R	R	R	R	S	R	S	S	+	ST1420
12b	22/06/2011	58 yr	F	Bronchial aspirate	32	R	R	R	R	R	S	S	S	−	ST1175
13c	22/07/2011	40 yr	M	Bronchial aspirate	32	R	R	R	R	S	S	R	S	−	ST1420
14	18/08/2011	54 yr	M	Wound	16	R	R	R	R	R	S	S	S	+	ST1420
15	27/08/2011	5 yr	M	Wound	12	R	R	R	R	S	S	R	S	+	ST1420
16d	10/09/2011	10 yr	M	Urine	16	R	R	R	R	R	R	R	S	−	ST654
17	27/08/2011	45 yr	M	Wound	12	R	R	R	R	R	S	S	S	+	ST1420

^aCAZ, ceftazidime; CIP, ciprofloxacin; COL, colistin; FOS, fosfomycin; GEN, gentamicin; TCC, ticarcillin-clavulanic acid; TIC, ticarcillin; TZP, piperacillin-tazobactam; M, male; F, female; R, resistant; S, susceptible. MICs were determined by the Etest.

^bContains a ₅₂₉CGA-to-TGA mutation, leading to a stop codon in the oprD gene.

^cExhibits a ₁₉₃TGGA-to-TG·A deletion, leading to a stop codon in the oprD gene.

^dContains a ₇₅₇TCG-to-TAG mutation, leading to a stop codon in the oprD gene.