Table 1.

Aminoglycoside susceptibilities of the K. pneumoniae clinical strains and E. coli experimental strains

Strain	Origin	MIC (μg/ml) ofa:											16S-RMTase	β-Lactamase	ST	Inc typeb
		GEN	TOB	AMK	ABK	NEO	APR	CAZ	CTX	FEP	ETP	MEM
K. pneumoniae 64/11	Tracheal aspirate	>256	>256	>256	>256	>256	8	128	>256	128	>32	>32	rmtD1	KPC-2, CTX-M-15	437	N, A/C
K. pneumoniae 368/10	Unknown	>256	>256	>256	>256	128	8	12	64	>256	>32	>32	rmtD2	KPC-2	11	A/C
K. pneumoniae 253/11	Rectal swab	>256	>256	>256	>256	32	8	8	256	>256	>32	>32	rmtD2	KPC-2	11	A/C
K. pneumoniae 145/11	Rectal swab	>256	>256	>256	>256	16	4	32	32	12	>32	32	rmtG	KPC-2, CTX-M-59, TEM-1	1046	N, L/M
K. pneumoniae 1194/11	Catheter tip	>256	>256	>256	>256	16	4	192	>256	48	8	4	rmtG	KPC-2, CTX-M-15, TEM-1	340	ND
K. pneumoniae 350/10	Bone	>256	>256	>256	>256	4	8	16	256	96	4	4	rmtG	CTX-M-2, TEM-1	442	ND
K. pneumoniae 84/11	Tracheal aspirate	>256	>256	>256	>256	16	2	>256	>256	32	>32	>32	rmtG	KPC-2, CTX-M-59, TEM-1	442	N, L/M
K. pneumoniae 922/11	Urine	>256	>256	>256	>256	16	8	>256	>256	>256	>32	>32	rmtG	KPC-2, CTX-M-2, TEM-1	442	N, A/C
E. coli DH10B(pKp350/10H3)		>256	>256	>256	>256	2	4						rmtG
E. coli DH10B(prmtG)		>256	>256	>256	>256	2	4						rmtG
E. coli DH10B(pKp84/11)		>256	>256	>256	>256	2	8						rmtG	CTX-M-59		N
E. coli DH10B[pBC-SK(−)]		1	0.5	2	1	2	8
E. coli DH10B		1	1	2	1	4	4

^aThe MICs of aminoglycosides were determined by the agar dilution method. The MICs of cephalosporins and carbapenems were determined by Etest (bioMérieux, Hazelwood, MO). GEN, gentamicin; TOB, tobramycin; AMK, amikacin; ABK, arbekacin; NEO, neomycin; APR, apramycin; CAZ, ceftazidime; CTX, cefotaxime; FEP, cefepime; ETP, ertapenem; MEM, meropenem.

^bND, not determined.