Table 7.
Viral titers from cloacal swab samples in individual ducks following transmission experiments
| Virus inoculum | Titer at post-virus inoculation daya: |
|||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
| 182742 wild type | ||||||||
| Duck 1 inoculated | 0.00 | 2.00 | 3.70 | 2.30 | 0.00 | 0.00 | 0.00 | 0.00 |
| Duck 2 inoculated | 0.00 | 1.90 | 3.30 | 2.00 | 1.00 | 1.90 | 1.85 | 0.00 |
| Duck 3 contact | 0.00 | 0.00 | 3.75 | 3.30 | 4.04 | 0.00 | 2.60 | 0.00 |
| Duck 4 contact | 0.00 | 0.00 | 3.58 | 1.85 | 3.48 | 2.00 | 1.78 | 0.00 |
| Duck 5 contact | 0.00 | 0.00 | 5.15 | 0.00 | 0.00 | 1.70 | 0.00 | 0.00 |
| Duck 6 contact | 0.00 | 0.00 | 0.00 | 3.30 | 3.30 | 2.00 | 0.00 | 0.00 |
| Duck 7 contact | 0.00 | 0.00 | 4.78 | 2.90 | 3.18 | 0.00 | 0.00 | 2.30 |
| Duck 8 contact | 0.00 | 0.00 | 0.00 | 4.60 | 3.48 | 1.90 | 0.00 | 0.00 |
| E119V resistant | ||||||||
| Duck 9 inoculated | 0.00 | 0.00 | 2.08 | 1.85 | 2.36 | 1.78 | 2.60 | 0.00 |
| Duck 10 inoculated | 0.00 | 0.00 | 1.85 | 2.54 | 3.70 | 1.70 | 1.85 | 0.00 |
| Duck 11 contact | 0.00 | 0.00 | 0.00 | 1.60 | 2.18 | 3.70 | 3.30 | 0.00 |
| Duck 12 contact | 0.00 | 0.00 | 0.00 | 0.00 | 1.70 | 3.48 | 2.60 | 0.00 |
| Duck 13 contact | 0.00 | 0.00 | 0.00 | 1.78 | 2.00 | 3.48 | 2.30 | 0.00 |
| Duck 14 contact | 0.00 | 0.00 | 0.00 | 0.00 | 3.70 | 2.85 | 3.00 | 0.00 |
| Duck 15 contact | 0.00 | 0.00 | 0.00 | 3.30 | 2.04 | 1.78 | 0.00 | 0.00 |
| Duck 16 contact | 0.00 | 0.00 | 0.00 | 0.00 | 3.18 | 4.30 | 0.00 | 0.00 |
| 1:1 mixture | ||||||||
| Duck 17 inoculated | 0.00 | 1.70 | 7.69 | 3.60 | 0.00 | 2.49 | 0.00 | 0.00 |
| Duck 18 contact | 0.00 | 0.00 | 0.00 | 3.18 | 2.23 | 3.30 | 2.60 | 0.00 |
| Duck 19 contact | 0.00 | 0.00 | 4.30 | 3.79 | 4.00 | 3.30 | 1.70 | 0.00 |
| Duck 20 contact | 0.00 | 0.00 | 0.00 | 3.30 | 3.48 | 3.90 | 2.60 | 0.00 |
| Duck 21 inoculated | 0.00 | 3.60 | 4.04 | 2.85 | 3.00 | 2.00 | 0.00 | 1.30 |
| Duck 22 contact | 0.00 | 0.00 | 4.08 | 3.30 | 2.36 | 3.78 | 0.00 | 0.00 |
| Duck 23 contact | 0.00 | 0.00 | 3.95 | 3.85 | 2.00 | 2.60 | 2.18 | 0.00 |
| Duck 24 contact | 0.00 | 0.00 | 3.70 | 3.78 | 3.30 | 0.00 | 0.00 | 0.00 |
| Duck 25 inoculated | 0.00 | 5.60 | 4.00 | 4.48 | 3.58 | 3.30 | 2.78 | 1.85 |
| Duck 26 contact | 0.00 | 0.00 | 0.00 | 4.34 | 3.00 | 2.08 | 2.70 | 0.00 |
| Duck 27 contact | 0.00 | 0.00 | 4.15 | 6.00 | 5.70 | 3.71 | 1.48 | 0.00 |
| Duck 28 contact | 0.00 | 0.00 | 3.49 | 4.20 | 4.34 | 2.78 | 2.60 | 0.00 |
a
Viral titers reported in log10 PFU/ml; values of 0.00 represent titers of <10 PFU/ml. Samples highlighted in bold were extracted for RNA, followed by RT-PCR and Sanger sequencing.