Table 1.
Antimicrobial activity of selected peptides (library PL-D)
| Sequence | MIC (μM)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| M. tuberculosis | M. smegmatis | P. aeruginosa | E. coli | S. enterica serovar Typhimurium | C. albicans | S. epidermidis | S. aureus | E. faecalis | |
| WKWLKKWIK | 1.1 | 1.9 | 2.9 | 5.8 | 11.5 | 5.8 | 0.7–0.3 | 0.7 | 11.5 |
| ILRWKWRWWRWRR | 2.4 | 2.4 | 5.7 | 1.4 | 2.9 | 5.7 | 0.7 | 0.7 | 2.9 |
| ILPWKWRWWKWRR | 2.6 | 2.2 | 5.7 | 1.4 | 5.7 | 2.9 | 0.7 | 0.7 | 2.9 |
| RWRRKWWWW | 2.8 | 5.7 | 11.2 | 1.4 | 2.8 | 5.6 | 0.7 | 0.7 | 2.8 |
| WRKFWKYLK | 3.0 | 1.5 | 6.0 | 0.8 | 6.0 | 3.0 | 0.8 | 0.8 | 6.0 |
| KRWWKWWRR | 3.1 | 4.9 | 11.1 | 2.8 | 5.6 | 5.6 | 0.7 | 0.7 | 11.1 |
| RRWWKWWWR | 3.6 | 4.8 | 5.4 | 2.7 | 2.7 | 5.4 | 0.7 | 0.7 | 5.4 |
| KIWWWWRKR | 4.1 | 5.4 | 42.3 | 5.3 | 10.6 | 10.6 | 1.3 | 1.3 | 10.6 |
| RLWWWWRRK | 4.1 | 2.4 | 16.6 | 4.1 | 8.3 | 4.1 | 0.5 | 2.1 | 4.1 |
| KWKWWWRKI | 4.2 | 6.4 | 4.6 | 4.6 | 4.6 | 4.6 | 1.2 | 1.2 | 4.6 |
| RIRRWKFRW | 4.3 | 1.4 | 42.6 | 5.3 | 5.3 | 21.3 | 1.3 | 1.3 | 5.3 |
| RLKRWWKFL | 4.5 | 3.0 | 9.8 | 1.2 | 4.9 | 9.8 | 1.2 | 2.4 | 9.8 |
| RWWRWRKWW | 4.6 | 4.6 | 10.3 | 2.6 | 5.2 | 2.6 | 0.6 | 0.6 | 5.2 |
| KRWWWWRFR | 5.2 | 5.2 | 46.9 | 2.9 | 5.9 | 5.9 | 0.7 | 0.7 | 11.7 |
| KRWWRKWWR | 5.3 | 5.3 | 43.2 | 2.7 | 5.4 | 5.4 | 0.7 | 0.7 | 10.8 |
| RRWWRWVVW | 5.6 | 11.4 | 2.6 | 0.7 | 2.6 | 10.4 | 0.7 | 1.3 | 2.6 |
| WFKMRWWGR | 5.9 | 2.2 | 46.1 | 5.8 | 23.1 | 11.5 | 2.9 | 2.9 | 11.5 |
| KFKWWRMLI | 6.1 | 15.3 | 4.6 | 0.6 | 2.3 | 4.6 | 0.6 | 1.2 | 2.3 |
| FIKWKFRWWKWRK | 7.4 | 3.7 | 6.0 | 1.5 | 1.5 | 3.0 | 0.7 | 0.7 | 1.5 |
| RIKRWWWWR | 7.8 | 4.6 | 86.3 | 2.7 | 2.7 | 5.4 | 1.3 | 1.3 | 10.8 |
| RWRWWWRVY | 8.0 | 6.7 | 13.2 | 1.6 | 3.3 | 13.2 | 1.6 | 3.3 | 3.3 |
| HQFRFRFRVRRK | 8.2 | 4.1 | 32.0 | 8.0 | 4.0 | 8.0 | 1.0 | 2.0 | 16.0 |
| LKRRWKWWI | 8.8 | 5.1 | 19.1 | 2.4 | 9.6 | 19.1 | 1.2 | 2.4 | 9.6 |
| RRRIKIRWY | 8.9 | 1.5 | 38.2 | 0.6 | 9.5 | 19.1 | 1.2 | 2.4 | 9.5 |
| RLWWKIWLK | 9.0 | 3.8 | 22.5 | 2.8 | 11.3 | 5.6 | 1.4 | 1.4 | 5.6 |
| KRRWRIWLV | 9.1 | 2.7 | 3.0 | 3.0 | 3.0 | 12.0 | 1.5–0.7 | 1.5 | 3.0 |
| FFIYVWRRR | 11.9 | 1.3 | 15.3 | 3.8 | 7.6 | 7.6 | 1.0 | 1.9 | 3.8 |
| IRMRIRVLL | 13.7 | 0.3 | 16.0 | 2.0 | 4.0 | 16.0 | 2.0 | 4.0 | 16.0 |
| RWWRKIWKW | 16.6 | 8.3 | 2.2 | 8.8 | 8.8 | 4.4 | 1.1 | 2.2 | 4.4 |
| LRFILWWKR | 17.0 | 4.2 | 25.7 | 3.2 | 6.4 | 6.4 | 1.6 | 1.6 | 6.4 |
| RWWIRIRWH | 17.0 | 1.6 | 10.7 | 1.3 | 2.7 | 10.7 | 1.3 | 1.3 | 2.7 |
| RRRWWKLMM | 17.6 | 2.2 | 11.0 | 2.7 | 11.0 | 21.9 | 2.7–1.4 | 5.5 | 21.9 |
| LRRWIRIRW | 17.7 | 2.2 | 21.4 | 0.7 | 5.4 | 10.7 | 1.3–0.6 | 1.3 | 5.4 |
| RKFRWWVIR | 17.8 | 3.7 | 21.9 | 2.7.0 | 5.5 | 21.9 | 1.4–0.5 | 1.4 | 10.9 |
| WKIVFWWRR | 23.3 | 4.4 | 21.9 | 1.4 | 2.7 | 5.5 | 0.7 | 1.4 | 2.7 |
| RQRRVVIWW | 24.7 | 1.9 | 10.7 | 1.3 | 5.3 | 21.4 | 1.3 | 2.7 | 5.3 |
| RRWRVIVKW | 24.7 | 2.3 | 2.7 | 1.3 | 2.7 | 5.4 | 0.7 | 1.3 | 2.7 |
| RRWKIVVIRWRR | 24.8 | 6.2 | 5.6 | 1.4 | 2.8 | 11.1 | 0.3 | 0.7 | 1.4 |
| RLWRIVVIRVKR | 25.9 | 4.3 | 7.1 | 1.8 | 3.5 | 7.1 | 0.4 | 0.9 | 1.8 |
| RLRRIVVIRVFR | 29.8 | 5.0 | 8.0 | 4.0 | 4. | 16.0 | 0.5 | 1.0 | 2.0 |
| VRLRIRVRVIRK | 30.1 | 1.6 | 2.0 | 2.0 | 2.0 | 8.0 | 1.0 | 1.0 | 4.0 |
| RRYHWRIYI | 33.2 | 16.6 | 12.5 | 1.6 | 6.3 | 12.5 | 3.1 | 3.1 | 12.5 |
| RKWKIKWYW | 34.5 | 2.9 | 5.2 | 0.7 | 2.6 | 10.5 | 1.3 | 2.6 | 5.2 |
| YRLRVKWKW | 36.0 | 1.5 | 14.3 | 3.6 | 7.1 | 3.6 | 0.9 | 1.8 | 7.1 |
| WKWRVRVTI | 51.5 | 0.8 | 5.4 | 1.3 | 2.7 | 10.8 | 1.3 | 2.7 | 2.7 |
| RTKKWIVWI | 78.1 | 9.8 | 11.6 | 1.4 | 5.8 | 23.1 | 2.9 | 5.8 | 11.6 |
| NWRKLYRRK | 109.3 | 38.3 | >86.5 | 86.5 | >86.5 | 86.5 | 43.3 | 86.5 | >86.5 |
| YKFRWRIYI | 130.0 | 16.2 | >92.8 | 2.9 | >92.8 | >92.8 | 1.4 | 2.9 | 2.9 |
| KRKKRFKWW | 141.0 | 8.8 | 165–80.3 | 20.6 | 20.6 | 41.2 | 2.6 | 2.6 | 10.3 |
| Median | 9.0 | 4.1 | 11.2 | 2.6 | 5.3 | 8.0 | 1.2 | 1.4 | 5.3 |
a
In vitro assays for M. tuberculosis and M. smegmatis are described in Materials and Methods and presented as MIC90 values. For all others, the MIC was defined by no visual growth. Peptides originating from PL-C are shown in bold.