Fig 2.

Csn-B inhibited the secretion of SPI-1 effectors in a dose-dependent manner and did not affect growth of S. enterica serovar Typhimurium. (A) Csn-B inhibited secretion of SPI-1 effector proteins at a range of concentrations from 6.25 to 100 μM under an SPI-1-induced condition in vitro. Culture supernatant proteins were subjected to 12% SDS-PAGE followed by Coomassie blue staining or Western blotting. M, marker; C, DMSO control. Inhibition percentage was calculated by comparing the SipC blot with that of the DMSO control. (B) Csn-B at a concentration of 100 μM did not significantly inhibit the bacterial growth.