Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| S. enterica serovar Typhimurium UK-1 χ8956 | ΔPrpoS183::TT araC pBAD rpoS | 47 |
| E. coli DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mk+) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| Plasmids | ||
| pKD46 | Vector containing the promoter of araC pBAD activator used in λ Red recombination, Apr | 50 |
| pWSK29 | Low-copy-no. vector, Apr | 51 |
| pBAD-hilA-pWSK29 | pWSK29 carrying araC pBAD and hilA, Apr | This work |
a
Apr, resistant to ampicillin.