Fig 5.
Colistin resistance assay. P. aeruginosa PAO1 (PCells) (A), PAO1ΔwspF (BCells) (B), and PAO1/plac-yhjH (DCells) (C) were cultivated at 37°C in ABTGC medium with 0, 0.25, or 2 μg of colistin ml−1. The OD600 was monitored for 10 h. Means and SD from triplicate experiments are shown. (D) Fast-kill assay of P. aeruginosa PAO1 (PCells), PAO1ΔwspF (BCells), and PAO1/plac-yhjH (DCells) by 4 μg of colistin ml−1. The proportion of dead bacterial cells was monitored by using the Live/Dead BacLight bacterial viability kits (Invitrogen) after 10 min of treatment. *, P < 0.01.