Table 2.

Splicing variants.

Novel AS Events	Carraro et al.	Carraro et al. (*)	NGS-Trex (N > 1)	NGS-Trex (N > 2)
Total	2865	1757 (**)	1641	683
Canonical	na	na	1245	539
RT-PCR validation	18	36	36
qRT-PCR	8	16	16

As described more in detail in the text, NGS-Trex treats splicing events from the "intron point of view" while in Carraro work splicing are classified from the exon perspective. This implies that one cassette-exon event is identified by NGS-Trex as two splicing sites. To compare data we corrected data to take into account this difference. Column marked with (*) shows the corrected values. (**) 1757 events derive from 530 AS donor/acceptor site usage + 487 exon skipping + 562 exon inclusion + 89×2 exon inclusion (each exon inclusion needs 2 introns). See also Figure 4 in Carraro paper for details.