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Nuclear localization of ATXN1[30Q]-D776 is critical for disruption of CF synapse elimination. A, Western blot of ATXN1 protein levels in cerebellar lysates. ATXN1[30Q]-D776/+ and ATXN1[30Q]-D776-K772T/+. B, 100× immunofluorescent images of ATXN1 localization at PC layer. C, Graph of average number of VGLUT2 synapses per PC soma (right axis, black bars) and number of PCs with >10 VGLUT2 synapses (left axis, red bars) in ATXN1[30Q]-D776-K772T and ATXN1[30Q]-D776/+ mice.
