Figure 4. Knockdown of MOG1 expression significantly decreased the PM expression of NaV1.5 without affecting the expression of total Nav1.5.
A. Relative amounts of Nav1.5 in the PM of HEK/Nav1.5 cells analyzed by WB analysis. Inputs, 1/50 of total cell extracts used for PM protein isolation; GAPDH, loading control. B. Relative amounts of SCN5A mRNA analyzed by qRT-PCR. C. Relative amounts of Nav1.5 protein in the total cell extracts analyzed by WB analysis.