Table 1.

Genes identified in the screening for quorum sensing regulation

Chromosome	Genes	Strand1	Transposon position (redundancy)2	Position of gene in operon	Orthologs3	C8-HSL-induced regulation4	Function or predicted function of the genes [compound]5
1	Bamb_1141	−	27	1/2	10	M	Heat shock protein Hsp20
1	Bamb_21729	−	750 (3)	–	30	M	Dihydrolipoamide dehydrogenase
1	Bamb_22979	+	1096	2/2	29	M	Sulfate transporter
			1158
1	Bamb_2378	−	91	–	29	R	Spermidine synthase-like protein (SpeE)
1	Bamb_2404	+	131	–	4	M	Hypothetical protein
1	Bamb_2520	−	235	5/6	30	R	Sulfate adenylyltransferase, large subunit (cysN)
1	Bamb_3128	+	−170	–	22	I	Hypothetical protein
2	Bamb_3350	+	572 (2)	6/6	30	R	Tryptophan synthase subunit alpha (trpA)
2	Bamb_3366	+	−30	–	18	I	Hypothetical protein
2	Bamb_45789	+	385	2/3	24	M	Hypothetical protein
2	Bamb_47269	+	103	1/4	10	I	Tryptophan halogenase (prnA) [pyrrolnitrin]
			273
			432 (4)
2	Bamb_51099	+	113	–	3	I	Hypothetical protein
2	Bamb_55359	−	−108	1/2	29	M	ElaB (protein of unknown function DUF883)
3	Bamb_5622	+	70	–	22	I	PRC-barrel domain-containing protein
3	Bamb_5911	+	229	2/2	0	I	LuxR family transcriptional regulator [enacyloxins]
3	Bamb_5925	−	1463	2/10	2	I	Beta-ketoacyl synthase [enacyloxins]
3	Bamb_6465	−	343	–	10	I	FAD linked oxidase domain-containing protein
3	Bamb_64696	−	367 (2)	4/4	1	I	Cyclic peptide transporter [occidiofungins]
			402
36	Bamb_64726,8	−	4578	1/4	70	I	Amino acid adenylation domain-containing protein [occidiofungins]
	Bamb_64767,8	−	10962	3/6	38		Amino acid adenylation domain-containing protein [occidiofungins]
3	Bamb_64777	−	204	2/6	1	I	Short-chain dehydrogenase/reductase SDR [occidiofungins]

¹The sign + or − refers to the DNA strand encoding the gene identified by the BLAST searches (http://www.burkholderia.com).

²The position of transposon is indicated in base pairs (bp) since the predicted translational start site; the number between brackets indicates how often the transposon was identified at the same insertion site.

³Orthologs indexed in the Burkholderia website (http://www.burkholderia.com).

⁴Effect of C₈-HSL on gene expression deduced from LacZ reporter assay; R, repression; I, induction; M, moderate effect.

⁵Function or predicted function listed in the Burkholderia website; if genes are included in clusters, the name of the resulting compound is indicated between square brackets.

⁶Bamb_6469 and Bamb_6472 belong to the same predicted operon.

⁷Bamb_6476 and Bamb_6477 belong to the same predicted operon.

⁸The BLAST search did not allow to discriminate Bamb_6472 from Bamb_6476 for this mutant; thus, both genes are indicated.

⁹Genes for which a putative cep box has been predicted.