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. 2013 Feb 7;22(10):1983–1993. doi: 10.1093/hmg/ddt051

Figure 4.

Figure 4.

Steady-state levels of mitochondrial transcripts in the absence of TWINKLE. (A) Northern blot analysis of mRNAs and rRNAs isolated from the heart tissue of control (L/L) and knockout (L/L, cre) mice at 4 weeks of age. The nuclear 18S rRNA was used as a loading control. (B) Northern blot analysis of mRNAs and rRNAs isolated from the heart tissue of control (L/L) and knockout (L/L, cre) mice at 16 weeks of age. The nuclear 18S rRNA was used as a loading control. (C) Quantification of steady-state levels of the mRNA and rRNA transcripts from wild-type (L/L) and knockout (L/L, cre) mice at different ages (age 4 weeks, n = 5 pairs of animals; 8 weeks, n = 3; 12 weeks, n = 4; 16 weeks, n = 3;). Error bars represent SEM; *P < 0.05, **P < 0.01, ***P < 0.001, Student's t-test. (D) Northern blot analysis of tRNAs isolated from the heart tissue of control (L/L) and knockout (L/L, cre) mice at 4 weeks of age. The nuclear 18S rRNA was used as a loading control. (E) Northern blot analysis of tRNAs isolated from the heart tissue of control (L/L) and knockout (L/L, cre) mice at 16 weeks of age. The nuclear 18S rRNA was used as a loading control. The autoradiographs shown in panels A and D, as well as those shown in panels B and E, originate from the same gel. Therefore, the 18S loading control is identical in those panels. (F) Quantification of steady-state levels of tRNAs from wild-type (L/L) and knockout (L/L, cre) mice at different ages (4 weeks, n = 5 pairs of animals; 8 weeks, n = 3; 12 weeks, n = 4; 16 weeks, n = 3). Error bars represent SEM; *P < 0.05, **P < 0.01, ***P < 0.001, Student's t-test.