Skip to main content
. 2013 Feb 7;22(10):2041–2054. doi: 10.1093/hmg/ddt055

Figure 3.

Figure 3.

Reduced glutamate uptake and enhanced neuronal excitability in fmr1−/− mice. (A) Immunoblot of GLT1 protein in crude synaptosome preparation from P40 cortex. Total (B) and GLT1-mediated (C) functional glutamate uptake levels in the cortex of fmr1−/− mice during post-natal development n = 6 mice. DHK: 500 μm; TBOA: 500 μm. *P < 0.05; **P < 0.01 from Student's t-test. (D) A representative example of firing rate in a layer 5 somatosensory neocortical neuron in fmr1+/+ (left) control and fmr1−/− (right) cortical slices before and during bath application of GLT1 inhibitor DHK (10 μm). Segment of same trace in fmr1−/− slice is shown in more expanded time scale. (E) Group data showing the effects of DHK on a neuronal firing rate in fmr1+/+ (n = 9) and fmr1−/− (n = 9) cortical slices with 10 and 50 μm DHK application. **P < 0.01, ****P < 0.0001 from one-way ANOVA with the Bonferroni post-test.