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. Author manuscript; available in PMC: 2013 Apr 23.
Published in final edited form as: Stem Cells. 2008 May 29;26(8):2104–2113. doi: 10.1634/stemcells.2008-0115

Figure 7. GFP expression in HSC from GFAP-cre/GFP mice.

Figure 7

Analysis of gene expression by freshly isolated and 5 day culture-activated primary HSC from adult mice. Realtime RT-PCR was used to evaluated GFAP (A), PPARγ (B), aquaporin-1 (AQP) (C), CK-19 (D), MPK (E), NCAM (F), CK-7 (G), AFP (H),α-SMA (I), collagen I α1 (J), S100A4 (K), and GFP (L) mRNA levels. Western blot analysis for CK-7, αSMA and GFAP (M). Cultures of HSC were stained with anti-GFP antibody on day 0 (N) and day 5 (O). (Original magnification X63, *p < 0.05 vs day 0)