Figure 7.
A–B. GRNi cells were differentiated and after 4 weeks, and subsequently FZD2 was overexpressed for 4 weeks concurrently with GRN loss. The cells overexpressing demonstrate greater cell number (A), and fewer dying cells (B), suggesting overexpression of FZD2 is neuroprotective in the context of GRN loss (n=3, error bars ± s.e.m., unpaired two-tailed Student’s t-test, p<0.05). C–D. In normal NHNP cells, FZD2 overexpression does not cause a change in proliferation as measured by PCNA immunoblotting (C) or by brdU immunostaining (D).