Table 4.

Selected IRF and STAT genes in RNA-Seq data from THP1 cells cultured under NG and HG conditions

Gene Name	RPKM in HG	RPKM in NG	Fold Change
IRF7	47.21	4.9	9.63
STAT2	40.85	16.06	2.54
IRF1	19.24	7.69	2.5
STAT1	53.83	28.74	1.87
IRF2	17.15	10.15	1.69
IRF9	62.42	19.74	1.61
IRF3	23.09	18.54	1.25
IRF5	40.24	34.19	1.18
IRF8	103.95	157.9	0.66

Transcription factors corresponding to the 3 highly enriched motifs (P < 1E-12 and enrichment fold > 3) are listed. The gene-level expression in THP1 grown under normal glucose (NG) and HG levels were measured by RNA-Seq with reads per kb per million (RPKM) normalization. Genes with RPKM normalized reads ≥1 in THP1 cells grown in either 1 condition and expression level fold change between HG vs. NG no less than1.5 are considered differentially expressed. IRF, interferon regulatory factor.