Table I.
Organism/Gene | Method | Forward Sequence (5′→3′) | Reverse Sequence (5′→3′) |
---|---|---|---|
R, GAPDH | PCR | TTCAATGGCACAGTCAAGGC | TCACCCCATTTGATGTTAGCG |
R, HIF-1α | PCR | CGAGCTGCCTCTTCGACAAG | CCCAGCCGCTGGAGCTA |
R, RANTES | PCR | TGCCCACGTGAAGGAGTATTT | TTCTTCTCTGGGTTGGCACAC |
H, GAPDH | PCR | AACCTGCCAAGTACGATGACATC | GTAGCCCAGGATGCCCTTGA |
H, HIF-1α | PCR | CTCAAAGTCGGACAGCCTCA | CCCTGCAGTAGGTTTCTGCT |
H, RANTES | PCR | TGCCCACGTGAAGGAGTATTT | TTCTTCTCTGGGTTGGCACAC |
H, RANTES-HRE-M3 | SDM | CTCAAAGACATTAAGATCTTTTCCCAAAGGTCGCTTAGCAAG | CTTGCTAAGCGACCTTTGGGAAAAGATCTTAATGTCTTTGAG |
H, RANTES-HRE-M2 | SDM | CCTGCAGAGGATCAAGACAGCGTCTGGACCTCGC | GCGAGGTCCAGACGCTGTCTTGATCCTCTGCAGG |
H, RANTES-HRE-M1 | SDM | GCACGTGGACCTCGATCAGCCTCTCCCACA | CATGGTACCTGTGGGAGAGGCTGATCGAGGTCC |
H, RANTES-AP-1-M | SDM | GTGCTTGGTCAAAGAGGAAACTGATACAGCCACTCTAGATGAGAGAGCAGTG | CACTGCTCTCTCATCTAGAGTGGCTGTATCAGTTTCCTCTTTGACCAAGCAC |
H, RANTES wt HRE1 consensus oligonucleotides | EMSA | GTGGACCTCGCACAGCCTCTCC | GGAGAGGCTGTGCGAGGTCCAC |
H, RANTES mutant HRE1 consensus oligonucleotides | EMSA | GTGGACCTCGATCAGCCTCTCC | GGAGAGGCTGATCGAGGTCCAC |
H, RANTES wt AP1 consensus oligonucleotides | EMSA | GAAACTGATGAGCTCACTCTAG | CTAGAGTGAGCTCATCAGTTTC |
H, RANTES mutant AP1 consensus oligonucleotides | EMSA | GAAACTGATACAGCCACTCTAG | CTAGAGTGGCTGTATCAGTTTC |
H, RANTES | ChIP | AAAGAGGAAACTGATGAGCT | GTACCTGTGGGAGAGGCT |
H, Human; R, rat; SDM, site-directed mutagenesis. Site-specific mutations are depicted in bold.