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. Author manuscript; available in PMC: 2013 Apr 23.
Published in final edited form as: Breast Cancer Res Treat. 2009 Jul 11;121(1):65–78. doi: 10.1007/s10549-009-0457-3

Figure 5.

Figure 5

Chemotherapeutic agents stimulate MT polymerization and McTN formation. MCF-10A, SkBr3 and MDA-436 cells were treated with 0.15% DMSO vehicle control (top row), 500nM jas (middle row), or a combination of jas and Taxol (bottom row, 1μg/mL) for 1 hour. Cells were suspended for 15 minutes, fixed, and gently spun onto glass coverslips. Cells were then labeled with an antibody against α-tubulin and analyzed by confocal microscopy. Three-dimensional volumes are presented (volume) as well as surface renders to indicate changes in cellular architecture (surface). Treatment with jas or jas+Taxol increased the extension of microtubules beyond the cell periphery in microtentacle protrusions (arrowheads).