Figure 7.

Role of lumican on the expression and distribution of N-WASP, cortactin and sm α-actin in prostate cancer cells. Coverslips placed in 24 well polystyrene culture dishes were incubated in a solution of either 1 µg/mL collagen I or sequential 1 µg/mL collagen I and 10 µg/mL lumican. After culture dish treatment, 2 × 10⁴ prostate cancer cells, PC3 (A) or DU145 (B), were seeded in RPMI containing L-glutamine/penicillin/streptomycin and 10% FBS, and incubated for 24 h at 37°C in a 5% CO₂ humidified environment. A scratch was performed in the cells on the culture dish using a 100 µL pipette tip. Cells were washed three times with EBSS, maintained for a further 4 h (37°C, 5% CO₂) and fixed for immunocytochemistry. The cells were labeled with anti-N-WASP, anti-sm α-actin, anti-cortactin and DAPI and analyzed using a Zeiss LSM510 scanning Confocal inverted microscope.