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Myocardial cytokine mRNA expression. Representative ribonuclease protection assays show expression of IL-1β, IL-6, and IFN-γ in subendocardial tissue. Lane 1, minimal expression in nonischemic myocardium; lane 2, stimulation of expression of all 3 cytokines by 90 min of low-flow ischemia (Isch) and 90 min of reperfusion (Reperf); lanes 3–5, attenuation of cytokine expression in ischemic-reperfused myocardium by pretreatment with troglitazone and α-tocopherol, but not rosiglitazone.
