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. Author manuscript; available in PMC: 2013 Apr 23.
Published in final edited form as: Cell Rep. 2013 Mar 14;3(3):646–650. doi: 10.1016/j.celrep.2013.02.016

Figure 1. Differentiation of Grafted Neural Progenitors.

Figure 1

(A) Schematic diagram of iPSC differentiation to neural progenitors and dopaminergic neurons.

(B) Quantification of cell populations at day 42 (the day for transplantation).

(C) On day 14, neuroepithelia organize into rosettes and express Pax6. By day 42, most cells express Tuj1, and some are positive for TH and GABA.

(D) Six months posttransplantation, Hoechst-labeled nuclei and corresponding GFP-labeled graft in the putamen show nondisrupted cytoarchitecture of the grafted area as well as the graft and cellular projections.

(E) Quantification of neurons (MAP2+), astrocytes (GFAP+), and oligodendrocytes (MBP+) among GFP+ grafted cells.

(F) Colabeling of GFP with neuronal (Tuj1, MAP2, NeuN, TH, GABA) and glial (GFAP, MBP) markers in the graft. HO, Hoechst. The bar represents 50 mm.

Data are presented as mean ± SE. See also Figure S1.