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. Author manuscript; available in PMC: 2013 Apr 23.
Published in final edited form as: J Immunol. 2008 May 1;180(9):6159–6167. doi: 10.4049/jimmunol.180.9.6159

Figure 3.

Figure 3

Heterologous immunization induces IgG1: IgG2a ratios indicative of a Th1-biased immune response. Groups of 5 female Balb/c mice were vaccinated with a human HBV vaccine, the indicated individual components of the immunization regimen. All immunizations were given at three week intervals. IgG1 to IgG2a ratios were determined from quantitative measurements of HBsAg-specific serum IgG1 and IgG2a measured two weeks after the last immunization on pools of sera from 5 mice. Grey bars represent the IgG1: IgG2a ratios from mice that received one immunization. Black bars are the ratios from mice that received two immunizations. Two vertical lines at 0.5 and at 2.0 demarcate three different patterns of antibody responses. A ratio of 0.5 or less indicates a Th1 polarized response. A ratio of 2.0 or more indicates a Th2 polarized response. Ratios between 0.5 and 2.0 indicate a mixed or balanced response.