Table 1.

HBsAg-specific antibody responses are enhanced by liposomes (Long protocol)

Group*	Prime	Boost	Ag-specific IgG (μg/ml)^	Comparison	p value
A1	HBsAg-DNA	None	23.9 ± 8.8	A1 vs. A2	<0.001
A2	HBsAg-DNA	HBsAg	109.5 ± 17.6	A2 vs. A3	<0.01
A3	HBsAg-DNA	HBsAg-liposome	374.8 ± 64.4	A3 vs. A1	<0.001
B1	HBsAg-DNA	None	28.6 ± 5.1	B1 vs. B2	<0.001
B2	HBsAg-DNA	HBsAg	203.4 ± 43.9	B2 vs. B3	<0.01
B3	HBsAg-DNA	HBsAg-liposome	618.0 ± 99.6	B3 vs. B1	<0.001

^*Two independent experiments A and B were performed with three sub-group comparisons in each experiment (1, 2, and 3).

^{^}HBsAg-specific serum IgG was evaluated using a quantitative ELISA assay on individual mouse serum samples. Experiments A and B contained 8 (N = 8) and 6 (N = 6) mice per group, respectively.