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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Immunogenetics. 2013 Feb 8;65(5):357–370. doi: 10.1007/s00251-013-0684-y

Fig. 3. Specificity of RM3 transfectant HLA expression.

Fig. 3

Expression of HLA class II MHC molecules in single transfected RM3 cell lines, as determined by HLA locus specific monoclonal antibodies. Expression was evaluated by flow cytometry using anti-DR (Panel a), -DP (Panel b), and –DQ (Panel c) antibodies (LB3.1, B7/21, and SPVL3, respectively). As a positive control, HLA Class I expression was evaluated using the anti-Class I antibody, W6/32 (Panel d). Black: LG2 EBV cell line (positive control); red: HLA DRB3*02:02 transfected RM3 line RRB3.02.2; green: HLA DQB1*06:02 transfected RM3 line RQ0602.3; blue: HLA DPB1*04:01 transfected RM3 line RP0401.2.