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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Immunogenetics. 2013 Feb 8;65(5):357–370. doi: 10.1007/s00251-013-0684-y

Fig. 4. Functional validation of transfected HLA: evaluation of peptide binding capacity.

Fig. 4

HLA class II molecules were purified from single transfected cell lines as described in the Materials and Methods, and then tested for their capacity to bind a panel of peptides representing a range of previously known affinities. The binding affinities of peptides in the panel (Y-axis) were compared to those obtained at the same time using MHC purified from homozygous EBV transformed lines (X-axis) available in the IHWG reference panel. Shown are affinities, expressed in terms of IC50 nM, obtained for four representative preparations. In each case the correlation between affinities measured using MHC from both sources was >0.81, comparable to the correlation observed when using two different preparations of the same molecule purified from the same EBV cell line.