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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Immunol. 2013 Mar 27;190(9):4640–4649. doi: 10.4049/jimmunol.1202312

Figure 1. Blockade of fatty-acid synthesis inhibits dendropoiesis in mice and humans.

Figure 1

(a–c) Mice were treated for four weeks with C75 or saline. (a) Live CD45+ liver leukocytes were gated using flow cytometry and the sub-fraction of hepatic CD11c+ cells was determined. (b) The percentage decrease in the number of liver, spleen, and bone marrow DC was calculated. (c) The fraction of splenocytes expressing CD3, CD19, and CD11b in saline- or C75-treated mice was tested. (d–g) BMDC were grown alone or with TOFA. (d) The fraction of PI+ cells was calculated on day 8 of culture. (e) Day 8 BMDC and T-BMDC were also tested for expression of Caspase 3, Cleaved Caspase 3, BCL-xL, Cyclin B1, and β-actin by Western blotting. (f) In addition, the total number and fraction of CD11c+ cells was calculated in day 8 BMDC and T-BMDC cultures. (g) Cellular proliferation was compared in day 8 BMDC and T-BMDC by pulsing with 3H-Thymidine. (h) moDC grown in control media and TOFA-enriched media were tested for HLA-DR and CD11c expression. Median fluorescence index (MFI) is indicated for each respective histogram (*p<0.05; **p<0.01; ***p<0.001).